EDTA-Na<sub>2</sub> - anticoagulant for hemolymph of australian red claw crayfish (cherax quadricarinatus)

Purpose: selection of anticoagulants based on EDTA-Na 2 and methods their use for working with the hemolymph Australian red claw crayfish (C. quadricarinatus) Materials methods. For study, different-sized males females australian red-clawed (Cherax quadricarinatus (Von Martens, 1868)) were used. was used as an anticoagulant, concentrationof which 4%. A ml syringe a 23G needle removal pre-washed anticoagulant remaining in cone (about 1/3 volume filled solution). The experiment is presented following series: 1. differences between total hemocytes number (THC) proportion granulocytes native treated small amount ; 2. content protein (THP) hemolymph; 3. changes THC immediately after sampling day later. To carry out work, samples 20 individuals used, while first two series, taken from each, one third. Syringes stored refrigerator at temperature 8.5 °C. Additionally, similar studies carried treatment multicomponent consisting 4 g , 3 sodium citrate, glucose 1 NaCl per 100 distilled water. Hemolymph ventral sinus. Three indicators determined: (THC), (THC). determined Goryaev chamber under light microscope. THP by refractometric method. Results. study revealed significant (p<0.05), are 32% more than hemolymph, can be explained uniform distribution all types sample. There no series. Studies showed low level hemocyte preservation (p<0.05) compared to selection, therefore, they not reflected work. Conclusion. data obtained indicate that there possibility using four percent when hemolymph. prevents formation gel rapid destruction hemocytes, promotes cells allows refractometer determining blood protein. contributes during day, practice. At same time, work should continue development storing transporting anticoagulant.